Composite

Part:BBa_K3596046:Design

Designed by: Xinyu Liu   Group: iGEM20_GreatBay_SZ   (2020-10-22)


BIOT mut 4D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2803
    Illegal PstI site found at 8393
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 8393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2393
    Illegal BglII site found at 2857
    Illegal BglII site found at 4658
    Illegal BamHI site found at 1073
    Illegal BamHI site found at 3182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2803
    Illegal PstI site found at 8393
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2803
    Illegal PstI site found at 8393
    Illegal NgoMIV site found at 1300
    Illegal NgoMIV site found at 6145
    Illegal AgeI site found at 1146
    Illegal AgeI site found at 1439
    Illegal AgeI site found at 2002
    Illegal AgeI site found at 4626
    Illegal AgeI site found at 6914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7920
    Illegal SapI.rc site found at 4262


Design Notes

no


Source

E. coli O157:H7, G. sulfurreducens

References